Technical improvement to prevent DNA degradation of enteric pathogens in pulsed-field gel electrophoresis.
نویسندگان
چکیده
This study used a modified pulsed-field gel electrophoresis (PFGE) method with HEPES as a running buffer to prevent electrophoresis-related DNA degradation of nine Salmonella enterica subsp. enterica serovar Ohio, seven Salmonella serovar Newport, and two enterohemorrhagic Escherichia coli (non-O157) strains. All strains yielded identifiable bands with this method in contrast to a commonly applied PFGE method using Tris buffer.
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ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 40 9 شماره
صفحات -
تاریخ انتشار 2002